"Cantonese Tunes on Mott Street" follows three Cantonese opera enthusiasts in New York: a Chinese immigrant from a Cantonese opera family, a Hong Kong immigrant who moved to New York as a child, and a Chinese refugee from Cuba. For them, Cantonese opera performances serve as a sanctuary.

The 92-year-old previous nightclub dancer Coby Yee decides to get back on stage again after joining the senior dance troupe Grant Avenue Follies. Together they go on a tour for the last time, bridging once isolated Chinese communities in the US, Cuba and China.