The Bride unwaveringly continues on her roaring rampage of revenge against the band of assassins who had tried to kill her and her unborn child. She visits each of her former associates one-by-one, checking off the victims on her Death List Five until there's nothing left to do … but kill Bill.

An assassin is shot by her ruthless employer, Bill, and other members of their assassination circle – but she lives to plot her vengeance.

新娘（乌玛·瑟曼饰）曾经是致命毒蛇暗杀小组（D．I．V．A．S）的一员，企图通过结婚脱离血腥的生活。但是她的前同僚（汉纳、刘玉玲、薇薇卡·A·福克斯、迈克尔·马德森等人扮演）以及所有人的老板比尔（大卫·卡拉丁饰）的到来破坏了这一切。“比尔，”新娘请求说，“我怀孕了，是你的孩子。”但是回答她的是“砰”的一声枪响！4年后她在一家医院醒来，就立刻开始着手一次从得克萨斯到冲绳、东京以及墨西哥的复仇之旅，为了一个目标她要大开杀戒。“当我到达目的地之后，我将杀死比尔。”

After being set-up and betrayed by the man who hired him to assassinate a Texas Senator, an ex-Federale launches a brutal rampage of revenge against his former boss.