Struggling to find his place in the world while juggling school and family, Brooklyn teenager Miles Morales is unexpectedly bitten by a radioactive spider and develops unfathomable powers just like the one and only Spider-Man. While wrestling with the implications of his new abilities, Miles discovers a super collider created by the madman Wilson "Kingpin" Fisk, causing others from across the Spider-Verse to be inadvertently transported to his dimension.

For Peter Parker, life is busy. Between taking out the bad guys as Spider-Man and spending time with the person he loves, Gwen Stacy, high school graduation cannot come quickly enough. Peter has not forgotten about the promise he made to Gwen’s father to protect her by staying away, but that is a promise he cannot keep. Things will change for Peter when a new villain, Electro, emerges, an old friend, Harry Osborn, returns, and Peter uncovers new clues about his past.